SpecCheck

Primer Pair

Forward Primer (5'→3')

Reverse Primer (5'→3')

TaqMan Probe (5'→3') · optional

Database

Specificity stringency

Min mismatches for specificity

Max amplicon size (bp)

Screen against species

Homo sapiensHuman · txid9606Mus musculusMouse · txid10090Rattus norvegicusRat · txid10116Macaca fascicularisCynomolgus monkey · txid9541

Other species (enter an NCBI TaxID)

Enter a forward and reverse primer, select the species to screen, and run a BLAST check. Off-targets are flagged non-specific only when both primers bind with few enough mismatches to prime — matching NCBI Primer-BLAST.